Description
GI360 Complete is an advanced, innovative, comprehensive and clinically-applicable stool profile, utilising multiplex PCR molecular technology, coupled with growth-based culture and ID by MALDI-TOF (>1200 bacteria; >200 yeasts can be identified), sensitive biochemical assays and microscopy to detect and assess the status of pathogens, viruses, parasites and bacteria that may be contributing to acute or chronic gastrointestinal symptoms and disease. GI360 Complete includes a broad range of stool chemistry markers to assess digestion/absorption, inflammation, immunology, short chain fatty acids and intestinal health markers including beta-glucuronidase.
Methodology:
The test is broken down into sections:
Commensal bacteria and GI Pathogens measured by multiplex PCR.
Gold standard microscopy of parasites, worms and yeasts.
Microscopy of red and white blood cells, muscle fibres, vegetable fibres, pollen and Charcot-Leyden Crystals.
Culturing of commensal and pathogenic bacteria
Macroscopic assessment of stool colour, consistency and presence/ absence of mucus.
Elastase and Calprotectin – BÜHLMANN fCAL® & fPELA® turbo assays
Lactoferrin, Lysozyme, sIgA – Elisa
Carbohydrates – Colormetric
Fat Stain – Sudan IV
SCFA – Gas Chromatography
Beta Glucuronidase – Quantitative endpoint enzymatic (RUO)
Occult Blood – Guaiac
Stool pH – ISE
Analytes measured:
% Acetate
% Butyrate
% Propionate
% Valerate
Acinetobacter junii
Actinobacteria
Actinomycetales
Aeromonas spp.
Akkermansia muciniphila
Alistipes onderdonkii
Alistipes spp.
ascaris lumbricoides
Bacilli class
Bacteroides fragilis
Bacteroides pectiniophilus
Bacteroides spp.
Bacteroides spp. & Prevotella spp.
Bacteroides stercoris
Bacteroides zoogleoformans
Balandtidium coli
Beta-glurcuronidase
Bifidobacterium spp.
Blastocystis spp.
Butyrate
Calprotectin
Campylobacter (C. jejuni
capillaria hepatica
capillaria philippinensis
Carbohydrates
Catenibacterium mitsuokai
Cestodes – Tapeworms
Charcot-Leyden Crystals
Chilomastix mesnili
Clonorchis sinensis
Clostridia Class
Clostridioides difficile (Toxin A&B)
Clostridium L2-50
Coprobacillus cateniformis
Costridium methylpentosum
Cryptosporidium (C. parvum and C. hominis)
Dialister invisus
Dialister invisus & megasphaera micronuciformis
Dientamoeba fragiis
Diphyllobothrium latum
Dipylidium caninum
Dorea spp
Dysbiotic Bacteria
Edwardsiella tarda
Elastase
Endolimax nana
Entamoeba coli
Entamoeba hartmanni
Entamoeba histolytica
Entamoeba histoytica/ entamoeba dispar
Entamoeba polcki
Enterobacteriaceae
Enterobius vermicularis
Enteromonas hominis
Enterotoxigenic E. coli (ETEC) lt&st
Escherichia coli O157
Escherichia spp.
Eubacterium biforme
Eubacterium hallii
Eubacterium rectale
Eubacterium siraeum
Faecalibacterium prausnitzii
Fasciola hepatical/Fasciolopsis buski
Fat stain
Firmicutes
Giardia duodenalis
Giardia duodenalis (AKA inestinalis & lamblia)
Heterophyes heterophyes
Hookworm
Hymenolepis diminuta
Hymenolepis nana
Imbalance Bacteria
Isospora belli
Lachnospiraceae
Lactobacillus ruminis & Pediococcus acidilctici
Lactobacillus spp.
Lactoferrin
lodamoeba bütschlii
Lysozyme
Macroscopic Appearance (Colour
Muscle fibres
Mycoplasma hominis
Nematodes – Round worms
Occult blood
Parabacteroides johnsonii
Parabacteroides spp.
Paragonimus westermani
Pentatrichomonas hominis
pH
Phascolartobacterium spp.
Plesiomonas shigelloides
Pollen
Proteobacteria
Protozoa
RBC microscopy
Retortanmonas intestinalis
Ruminococcus albus & R. bromii
Ruminococcus gnavus
Salmonella group
Salmonella spp.
Secretory IgA
Shiga-like toxin-producing E. coli (STEC) stx1&stx2
Shigella (S. boydii
Shigella group
Sreptocococcus spp.
Strepococcus salivarius ssp. thermophilus
Streptococcus agalactiae & Eubacterium rectale
Streptococuss salivarius ssp. thermophilus & S. sanguinis
Strongyloides stercoralis
Taenia
Total SCFA’s
Trematodes – Flukes
Trichuris trichiura
Vegetables fibres
Veillonella spp.
Vibrio cholerae
Vibrio spp.
Viruses (adenovirus F40/41
WBC microscopy
Yeast
Yeast culture
Yersinia spp.
Please read all of the instructions carefully before beginning.
Patients can collect stool over 1, 2, or 3 days.
